The PET18 locus of Saccharomyces cerevisiae: a complex locus containing multiple genes

The basis of pleiotropy shown by the pet18 mutants of Saccharomyces cerevisiae (rho-0,KIL-0 and temperature sensitive growth) was examined by cloning the fragment which complements the defect in growth at 37 degrees C of the pet18 mutants. The cloned DNA could complement the defect in the maintenance of the killer plasmid but did not give the cell the ability to maintain mitochondrial DNA. Sequence analysis of the cloned DNA revealed the presence of four open reading frames, at least two of which are necessary for the complementation activity. By using the cloned DNA as a probe, we found that two independent pet18 mutants have a deletion covering the entire sequence contained in the probe. From these results we predict that the traits of the pet18 mutants that concern temperature sensitivity and killer of the pet18 mutants are controlled by a separate gene(s) from that which participates in the maintenance of mitochondrial DNA.     

XIV. Yeast sequencing reports. Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae

MKT1 is required for m aintenance of K₂ above 30°C in strains with the L-A-HN variant of the L-A double-stranded RNA virus of Saccharomyces cerevisiae. We report that MKT1 encodes a 92 979 Da protein with serine-rich regions and the retroviral protease signature, DTG, but with no substantial homology to proteins presently in the databases. This sequence is available from GenBank under Accession Number U09129.     

三相单级全桥PFC电压尖峰机理分析与抑制

介绍了一种基于全桥结构的三相单级功率因数校正(PFC)变换器,该变换器工作于电感电流断续模式(DCM),电感电流即输入电流的峰值自动跟踪输入电压,可实现功率因数校正.建立了桥臂开关对臂导通时变换器的简化模型,并分析了该阶段变压器原边电压尖峰的产生机理.分析结果表明,由于变压器漏感的存在,变压器原边于桥臂开关对臂导通期间将产生很大的电压尖峰,该电压尖峰大小由变压器原边的漏感、电流与并联电容三个参数决定.最后,结合电路的工作原理,提出了一种变压器原边无源缓冲电路来抑制该电压尖峰.实验结果表明,变压器原边的电压尖峰得到了有效抑制.     

Analysis and reduction of data spikes in thin client computing

While various optimization techniques have been used in existing thin client systems to reduce network traffic, the screen updates triggered by many user operations will still result in long interactive latencies in many contemporary network environments. Long interactive latencies have an unfavorable effect on users’ perception of graphical interfaces and visual contents. The long latencies arise when data spikes need to be transferred over a network while the available bandwidth is limited. These data spikes are composed of a large amount of screen update data produced in a very short time. In this paper, we propose a model to analyze the packet-level redundancy in screen update streams caused by repainting of graphical objects. Using this model we analyzed the data spikes in screen update streams. Based on the analysis result we designed a hybrid cache-compression scheme. This scheme caches the screen updates in data spikes on both server and client sides, and uses the cached data as history to better compress the recurrent screen updates in possible data spikes. We empirically studied the effectiveness of our cache scheme on some screen updates generated by one of the most bandwidth-efficient thin client system, Microsoft Terminal Service. The experiment results showed that this cache scheme with a cache of 2M bytes can reduce 26.7%–42.2% data spike count and 9.9%–21.2% network traffic for the tested data, and can reduce 25.8%–38.5% noticeable long latencies for different types of applications. This scheme costs only a little additional computation time and the cache size can be negotiated between the client and server.     

一种改进型含吸收回路的准△源升压变换器

针对传统耦合电感网络变换器中漏感所导致的母线电压尖峰问题,在△源变换器的基础上,结合LCD吸收回路与箝位二极管的概念,提出具有母线电压尖峰吸收能力的准△源变换器电路拓扑。分析了传统△源变换器电压尖峰的产生原理,并将吸收回路对电压尖峰的抑制进行理论分析。与传统的耦合电感网络变换器的拓扑结构相比较,所提拓扑大大提高了升压能力,有效抑制了母线电压尖峰,降低了元器件的电压应力。运用MATLAB/Simulink软件对电路进行了仿真,并在仿真的基础上搭建了实验样机,实验结果与理论分析相一致,验证了改进型电路拓扑的可行性和实用性。     

一种实时自适应前沿消隐电路

基于正激变换器结构,设计了一种实时自适应前沿消隐电路。通过振荡时间消隐和振荡幅度消隐,对最优消隐时间进行追踪,实现实时自适应的前沿消隐,在保证消隐可靠性的基础上降低了系统体积与成本,并确保不限制电路高频化发展。设计的前沿消隐电路能获得消除尖峰振荡的输出电流信息,保证系统能对输出负载变化做出快速响应。基于0.18 μm BCD工艺,对电路进行设计。仿真结果表明,该电路在没有其它额外限流功能条件下,启动过冲电压只有50 mV,负载切换时的下掉电压与过冲电压分别为519 mV和578 mV。     

基于钉板分布稀疏变分自编码器的异常检测算法研究

异常检测由于其广泛的应用一直是数据挖掘中一个重要的研究分支,它有助于研究人员获得重要的信息进而对数据做出更好的决策。提出了一种基于钉板分布稀疏变分自编码器的异常检测模型。首先,使用离散-连续混合模型钉板分布作为变分自编码器的先验,模拟隐变量所在空间的稀疏性,得到数据特征的稀疏表示;其次,以所提出的自编码器构建深度支持向量网络,对特征空间进行压缩,并采用最优超球体区分正常数据和异常数据;再次,以数据特征和超球体中心之间的欧氏距离完成异常检测;最后,在基准数据集MNIST （modifiednational institute of standards and technology database）和Fashion-MNIST上的实验评估表明,与现存的异常检测算法相比,本文所提出的算法具有更好的检测效果。     

Evaluation of thermal spike due to exothermic reaction in curing process of carbon fiber-reinforced plastic pressure accumulators for hydrogen stations

In the curing process of fiber-reinforced plastic accumulators, a thermal spike appears due to the exothermic reaction of the resin. It is necessary to accurately evaluate this thermal spike to guarantee quality and avoid using a trial and error approach to control the temperature. Thus, we developed a curing process simulator that is able to accurately evaluate the thermal spike of the thermosetting resin. A simplified mesoscale finite element model with the definite separation of fiber and resin is used in the simulation. In this study, the newly developed simulation system was examined by comparing its results with the experimental results from the curing process of fiber-reinforced plastic accumulators.     

兴奋性化学突触耦合的神经元的同步

基于动力系统的稳定性理论、数值计算分岔图和线性化系统的最大Lyapunov指数,研究了经兴奋性化学耦合的快峰神经元的同步动力学.研究表明,随着一些关键参数的改变,耦合神经元能呈现丰富的同步行为,如各种周期的同步和混沌的同步.研究结果对理解神经元系统的同步运动具有指导意义.     

约束稀疏脉冲反演在储层预测中的应用

约束稀疏脉冲反演包括测井和地震资料预处理、合成记录标定、建立初始波阻抗约束模型、选择反演参数以及频谱补偿等关键环节。以胜利油田C工区为例,该区储层砂砾岩体的横向变化大、尖灭点位置不明、测井资料分布不均。经约束稀疏脉冲反演得到的反演剖面上,横向上砂砾岩体的尖灭点反映清晰,纵向上波阻抗变化与测井岩性解释剖面吻合。